Update. The question has been recently answered in the positive by David Marker and Charles Steinhorn (as in indicated in Marker's answer). Note that Remark 3 below is now expanded by reference to a paper of Mekler and Shelah.
Question. Is there a countable rigid non-Archimedean real closed field?
Background:
As usual, a structure is said to be rigid if the only automorphism of the structure is the identity map.
It is well-known that any Archimedean ordered field is rigid; the argument is the same as that used by Darboux in 1880 to prove that the field of real numbers is rigid. Note that the Archimedean ordered fields are precisely those ordered fields that are isomorphic a subfield of the field of real numbers.
In 1983 Shelah showed that its consistent with the axioms of set theory (ZFC) that there is an uncountable rigid non-Archimedean closed field. Here is the reference: *Shelah, Saharon, Models with second order properties. IV. A general method and eliminating diamonds. Ann. Pure Appl. Logic 25 (1983), no. 2, 183–212. A decade later Mekler & Shelah in this paper showed in ZFC the compactness of the extension of first order logic by a quantifier over isomorphisms between (definable) ordered fields; as explained on page 2 of the article, this result can be used to show the existence, within ZFC, of arbitrarily large rigid RCFs (thanks to Biran Falk-Dotan for the information about the Mekler-Shelah paper).
Using machinery from the metamathematics of arithmetic, one can build (many) countable rigid non-Archimedean ordered fields. More specifically: it is well-known that countable nonstandard rigid models of PA exist (this is independently due to Gaifman and Ehrenfeucht). Let $\cal{M}$ be such a model, and let $\mathbb{Q}^{\cal{M}}$ be the field of rationals as computed in $\cal{M}$. By a classical theorem of Julia Robinson, $\cal{M}$ and $\mathbb{Q}^{\cal{M}}$ are bi-interpretable, therefore $\mathbb{Q}^{\cal{M}}$ is also rigid. One can use the same type of argument to show that there are (many) rigid non-Archimedean ordered fields in every infinite cardinality.