Let $R$ be an integral domain and $M_n(R)$ the ring of $n\times n$ matrices over $R$.
Question 1: Is there a ring homomorphism $f:R\rightarrow M_n(R)$ such that $f(r)$ is a nondiagonal matrix for some $r\in R$?
Question 2: Can such a homomorphism be chosen injective?
Clearly, both answers are "no" for $R=\mathbb{Z}$ (the only homomorphism is $f: z\mapsto zI$ where $I$ is the identity matrix).
There is an example where both answers are "yes": Let $R=\mathbb{Q}[x]$. Let us write elements of $R$ (uniquely) in the form $p+q$ where $p$ is in the ideal $(x)$ of $R$ and $q\in \mathbb{Q}$. Define $f:p+q\mapsto pX+qI$ where $X\neq I$ is a fixed idempotent matrix over $\mathbb{Q}$ (e.g. $X=\frac{1}{n}J$ where $J$ is the all-ones matrix). Then it is easy to see that $f$ is an injective ring homomorphism and $f(x)=xX$ is nondiagonal.
Can the questions be answered (positively or negatively) for some larger classes of integral domains?
My special interest is in rings $R$ that are models of (Peano or weaker) arithmetic. For this purpose one can assume some additional properties of $R$, e.g. $\pm 1$ are the only invertible elements, prime decompositions are unique when they exist (but they do not have to exist), $R$ is discretely ordered, all elements from the real closure have the unique integer part in $R$, ....