Let $L$ be the Laplacian matrix for a simple graph $G$ of $n$ vertices, and $\lambda_0,\ldots,\lambda_{n-1}$ its $n$ eigenvalues.
Q. What is the cardinality of the class of $n$-vertex graphs $\cal G$, that each have the same Laplacian eigenvalues as $G$? Presumably $G$ is not uniquely determined by its eigenvalues. What are examples of pairs or clusters of graphs with the same eigenvalues?
The smallest and second-smallest eigenvalues are the spectral gap and algebraic connectivity of $G$ respectively, and the third-smallest relates to partitioning $G$ into pieces. See the 2015 MO question, Spectral theory of graph Laplacian besides $\lambda_2$, for further properties of the eigenvalues.
I also wonder if the new result that eigenvectors are determined by eigenvalues might play a role here.
Natalie Wolchover. "Neutrinos Lead to Unexpected Discovery in Basic Math." Quanta. 2019.
Peter B. Denton, Stephen J. Parke, Terence Tao, Xining Zhang. "Eigenvectors from Eigenvalues." arXiv abstract. 2019.
Van Mieghem, Piet. "Graph eigenvectors, fundamental weights and centrality metrics for nodes in networks." arXiv abstract. 2014.