This question came up in our algebraic topology class and our Professor didn't know the answer. I also couldn't find an answer so far.
What is the cardinality of the set of subgroups of $F_2$?
Here $F_2 = \mathbb Z * \mathbb Z$ denotes the free group on two generators. The cardinality of the set of subgroups is clearly bounded below by $\aleph_0$ (as $F_2$ contains subgroups of all countable ranks) and above by $2^{\aleph_0}$.