I believe that the answer is NO. If you look at
Gutiérrez, Mauricio A.; Ratcliffe, John G. On the second homotopy group. Quart. J. Math. Oxford Ser. (2) 32 (1981), no. 125, 45–55.
Corollary 3 states that a "reduced 2-complex $K(X; R)$ is aspherical if and only if each element of $R$ is independent and not a proper power."
Now, "reduced" means that there is (a) only one 0-cell (true in your case), and the one cells represent distinct nontrivial elements of $\pi_1(K^1),$ where $K^1$ is the one-skeleton. Again seems to be true under your assumptions. $R$ are the relations (given by attaching maps of the 2-cells, I imagine), "independent" is too complicated to explain here (look at the paper), but in any case, the "not a proper power" condition is easy to violate.