The following represents what I know about this; I don't know of a published reference.

Given a map $f:Z_\bullet\to Y_\bullet$ of simplicial "spaces" (to make things easy, assume spaces are simplicial sets), let's call it a realization quasi-fibration (RQF) if for every $U_\bullet\to Y_\bullet$, the homotopy pullback of the geometric realizations is weakly equivalent to the realizations of the levelwise homotopy pullbacks.  The Bousfield-Friendlander theorem gives a sufficient condition for $f$ to be a  RQF, in terms of the dreaded $\pi_*$-Kan condition.

Some facts:

* The pullback of an RQF $f$ along any $U_\bullet\to Y_\bullet$ is itself an RQF.

* Let $F[n]_{\bullet}$ be the simplicial space which is free on a point in degree $n$.  Then $f$ is an RQF if and only if its pullback along all $g: F[n]_\bullet \to Y_\bullet$, for all $n$, is an RQF.

These two facts are consequences of something that is sometimes called "descent"; basically, the facts that homotopy colimits distribute over homotopy pullbacks, and compatible homotopy pullbacks assembled by a homotopy colimit result in a homotopy pullback.

So the above gives exact criteria for $f$ to be an RQF.  Whether the pullback of an  RQF $f$ along a map $g$ is again an RQF only depends on the homotopy class of $g$.  So if $f:Z_\bullet\to Y_\bullet$ is any map, let 
$\pi_0Y$ be the simplicial set whose $k$-simplices are $\pi_0(Y_k)$, which is to say all homotopy classes of maps $F[k]_\bullet\to Y_\bullet$.  Let $RQF(f)\subseteq \pi_0Y$ be the sub-simplicial set whose $k$-simplices correspond to $g:F[k]_\bullet\to Y_\bullet$ such that the pullback of $f$ along $g$ is an RQF.  

So the criterion is: $f$ is an RQF iff $RQF(f)=\pi_0Y$.

It turns out that since geometric realization always preserves products, any map $Z_\bullet \to point_\bullet$ is an RQF.  Thus $RQF(f)$ contains all $0$-simplices of $\pi_0Y$.  Thus, if all $Y_k$ are connected, $f$ is an RQF, which implies the result you describe.