Let $f \in C^{\infty}([0, 2\pi])$ be a smooth function and consider the following periodic Sturm-Liouville problem: $$\begin{cases} u''(x) + f(x)u(x) = - \lambda u(x) \\ u(0) = u(2\pi) \\ u'(0) = u'(2\pi) \end{cases}$$ I would like to know if there are mild sufficient conditions on $f$ so that the above problem has a negative simple eigenvalue $\lambda$.