See <A HREF="https://arxiv.org/abs/0801.1730">Extreme Value Statistics of Eigenvalues of Gaussian Random Matrices</A> (2008), in particular the large-$n$ result:

$$\text{Prob}(E_{\rm smallest}\geq x)=\exp\left[-N^2\Phi\left(\frac{x+\sqrt{2n}}{\sqrt{n}}\right)\right],\;\;-\sqrt{2n}<x<0,$$
$$\Phi(z)=S(-\sqrt{2})-S(-\sqrt{2}-z),$$
$$S(z)= \frac{1}{216}\left[ 72 z^2 -2z^4 +(30 z + 2z^3)
\sqrt{6 +z^2}+ 27\left( 3 + \ln 1296 - 4
\ln\left(-z + \sqrt{6 +z^2}\right)\right)\right].$$

The probability that all eigenvalues are positive follows from $x\rightarrow 0$, 

$$\text{Prob}(E_{\rm smallest}\geq 0)=3^{-N^2/4}.$$