Let $X$ be an infinite set, and let ${\cal E}$ be a collection of non-empty subsets of $X$. We say that ${\cal E}$ has property $\mathbf{B}$ if there is $B\subseteq X$ such that $B\cap E\neq \emptyset$ and $E\not\subseteq B$ for all $E\in{\cal E}$. (This is equivalent to saying that the hypergraph $(X,{\cal E})$ has chromatic number $2$.)
It is possible to find a chain ${\frak E}$ (with respect to $\subseteq$) of collections with property $\mathbf{B}$ such that $\bigcup {\frak E}$ has not property ${\mathbf B}$ - but this does not imply that not every set of subsets of $X$ with property ${\mathbf B}$ is not in a maximal such set of subsets. This leads to the following:
Question. What is an example of a set $X$ and and set of non-empty subsets ${\cal E}$ of $X$ with property ${\mathbf B}$ such that ${\cal E}$ is not contained in a set of non-empty subsets ${\cal E'}$ of $X$ that is maximal with respect to having property ${\mathbf B}$ and set inclusion?