Let $X$ and $Y$ be two path-connected $n$-dimensional manifolds. Then one can construct their connected sum $X\# Y$ which can be visualised as removing a disc from $X$, removing a disc from $Y$, and gluing the boundaries together using a cylinder (a copy of $S^{n-1}\times[0,1]$).
There is a natural $(n-1)$-sphere in $X\# Y$ due to the cylinder. If $X$ or $Y$ is a sphere, then this $(n-1)$-sphere will bound. Is this the only case in which it bounds?
If $X\# Y$ is a non-trivial connected sum (i.e. neither $X$ nor $Y$ is a sphere), is the map $S^{n-1} \to X\# Y$ essential (i.e. not nullhomotopic)?
Oscar Randal-Williams' answer to this question shows that the inclusions of the sphere into $X\setminus D^{\circ}$ and $Y\setminus D^{\circ}$ are essential.
If the answer is yes, then $\pi_{n-1}(X\# Y) \neq 0$. I don't even know if this is true.
If $X \# Y$ is a non-trivial connected sum of $n$-dimensional manifolds, is $\pi_{n-1}(X\# Y)$ necessarily non-zero?
I believe the answer to both of these questions is yes, but I have not been able to completely convince myself.