When does a finite morphism induce isomorphism on cohomology? Let $Y \to X$ be a finite morphism of schemes of dimension $n$.  The induced map on the top cohomology $H^n(X, \mathcal O_X) \to H^n(Y, \mathcal O_Y)$ is always surjective.  When is it injective?  Can one achieve this by adding reasonable conditions?  We may restrict ourselves to projective varieties over an algebraically closed field.
 A: I don't think that map is always surjective.  For example, suppose that $Y = X$ is a supersingular elliptic curve.  Then the Frobenius map $H^1(X, O_X) \to H^1(Y, O_Y)$ is the zero map (and both are 1-dimensional vector spaces).  See for example Hartshorne's chapter on elliptic curves.  
Alternately, suppose that $X$ is a scheme over $\mathbb{R}$, and $Y = X \times_{\mathbb{R}} \mathbb{C}$ is the base change.  I don't think one should expect that $H^n(X, O_X) \to H^n(Y, O_Y)$ is basically ever surjective unless they are both zero...
With regards to your question though, here's one answer:  
Suppose that $X$ is a normal integral scheme of characteristic zero and $Y$ is also integral.  Then the natural map $O_X \to f_* O_Y$ splits as a map of $O_X$-modules, say with splitting map $\phi : f_* O_Y \to O_X$ (use the trace map on the fields $K(Y) \to K(X)$ and restrict to the structure sheaves).  Now apply the functor $H^n(X, \bullet)$ to the composition (which is an isomorphism):
$$
O_X \to f_* O_Y \xrightarrow{\phi} O_X.
$$
Clearly one gets that
$$
H^n(X, O_X) \to H^n(X, f_* O_Y) = H^n(Y, O_Y) \to H^n(X, O_X)
$$
is also an isomorphism and thus
$$
H^n(X, O_X) \hookrightarrow H^n(Y, O_Y)
$$
injects as desired.

EDIT
Since the author of the question is particularly interested in the case when $f : Y \to X$ is the normalization of an $n$-dimensional $X$, let me try to say a couple things about that case.
Since we have a short exact sequence $0 \to O_X \to f_* O_Y \to C \to 0$, and since $f$ is birational, the support of $C$ has dimension $< \dim X$.  Therefore, $H^n(X, O_X) \to H^n(Y, O_Y)$ is surjective as the original question states.  
To show injectivity, it is sufficient to show that $H^{n-1}(X, C) = 0$.  This will happen certainly if the non-normal locus of $X$ has codimension $\geq 2$.  Otherwise, it generally won't happen.

A simple example with curves
Suppose that $X$ is a curve with exactly a node and $Y$ is its normalization (although any singular curve will work).  Then $C$ is the skyscraper sheaf supported at a point.  In particular $\dim H^0(X, C) = 1$.  On the other hand, $\dim H^0(Y, O_Y) = 1 = \dim H^0(X, O_X)$, and so the exact sequence
$$
0 \to H^0(X, O_X) \to H^0(Y, O_Y) \to H^0(X, C) \to H^1(X, O_X) \to H^1(Y, O_Y) \to 0
$$
immediately implies that $H^0(X, C) = \ker H^1(X, O_X) \to H^1(Y, O_Y)$.  In particular, the latter map is not injective.
