The question is simply: let $|S| = n,$ how big can a subset $I$ of $2^S$ be such that for any $A, B \in I,$ $A\not\subset B$ (so, this is an independent set in the graph whose vertices are subsets, and edges correspond to inclusion in some direction). It is clear that $|I| \geq \binom{n} {\lfloor \frac{n}{2} \rfloor},$ but is that sharp?
Update As Gerhard points out, the answer is YES. Now a followup: what about a family which has no $k$-element chain $A\subset B \subset \dotsc \subset C?$