One generalization with a spectral graph theory flavor is the Colin de Verdière Conjecture, originating in
Colin de Verdière, Yves. "Sur un nouvel invariant des graphes et un critere de planarité." Journal of Combinatorial Theory, Series B 50, no. 1 (1990): 11-21. Journal link (English translation in this volume)
For a graph $G$ with $n$ vertices, consider an $n\times n$ symmetric matrix $M$ satisfying:
- For every $i\neq j$, $M_{ij} < 0$ if $\{i,j\}$ is an edge in $G$, and $M_{ij} = 0$ otherwise.
(This looks a bit like a generalization of the usual graph Laplacian matrix.)
The smallest eigenvalue of $M$ must (by Perron-Frobenius) have multiplicity equal to the number of connected components of $G$. So what about the multiplicity of the second smallest eigenvalue? Now this may depend on the choice of $M$, so let's consider its maximum over all such matrices $M$.
Since the diagonal of $M$ is unconstrained, we may assume that this second-smallest eigenvalue is $0$. Then we're asking about the largest possible corank of such a matrix $M$.
Finally, require of $M$ a sort of nondegeneracy condition, called the Strong Arnold Property:
- Within the space of $n\times n$ real symmetric matrices, the submanifold comprising those that satisfy the bulleted condition above and that comprising those with the same rank as $M$ intersect transversally at $M$.
(A theorem of van der Holst, Lovász and Schrijver gives an equivalent algebraic condition: The only symmetric matrix $X$ with $MX=0$ that is zero on the diagonal and on the edges of $G$ is $X=0$.)
The largest corank of a matrix $M$ satisfying both of the bulleted conditions above is the Colin de Verdière number of $G$, denoted $\mu(G)$. This parameter has some nice properties, e.g., it is monotonic with respect to graph minors.
Most remarkably, Colin de Verdière showed that $\mu(G) \le 3$ if and only if $G$ is planar (and that $\mu(G) \le 2$ iff $G$ is outerplanar) and put forward
The Colin de Verdière Conjecture: $\chi(G) \le \mu(G)+1$
Currently, the conjecture is known to hold for $\mu(G) \le 4$. This relies on current proofs of the 4-color theorem, of course, although a direct proof of the conjecture could conceivably offer a very different route to that result.
ADDED: Colin de Verdière showed $\mu(G)=n-1$ iff $G=K_n$. (This seems obvious, but does require checking the Strong Arnold Property.) Together with the minor-monotonicity mentioned above, this shows that the conjecture would follow as a special case of the Hadwiger Conjecture, as pointed out here in a comment by Gil Kalai. This is also noted by Colin de Verdière himself upon stating the conjecture!