I'm not sure whether this is non-trivial or not, but do there exist simple examples of an affine scheme X$X$ having an open affine subscheme U$U$ which is not principal in X$X$? By a principal open of X = Spec A$X = \mathrm{Spec} \ A$, I mean anything of the form D(f) = {P in Spec A : f is not in P}$D(f) = \{\mathfrak p \in \mathrm{Spec} \ A : f \notin \mathfrak p\}$, where f$f$ is an element of A$A$.