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Theorem (ZFC + "There exists a supercompact cardinal."): There is no largest cardinal.

Proof: Let $\kappa$ be a supercompact cardinal, and suppose that there were a largest cardinal $\lambda$. Since $\kappa$ is a cardinal, $\lambda \geq \kappa$. By the $\lambda$-supercompactness of $\kappa$, let $j: V \rightarrow M$ be an elementary embedding into an inner model $M$ with critical point $\kappa$ such that $M^{\lambda} \subseteq M$ and $j(\kappa) > \lambda$. By elementarity, $M$ thinks that $j(\lambda) \geq j(\kappa) > \lambda$ is a cardinal. Then since $\lambda$ is the largest cardinal, $j(\lambda)$ must have size $\lambda$ in $V$. But then since $M$ is closed under $\lambda$ sequences, it also thinks that $j(\lambda)$ has size $\lambda$. This contradicts the fact that $M$ thinks that $j(\lambda)$, which is strictly greater than $\lambda$, is a cardinal.

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Theorem (ZFC + "There exists a supercompact cardinal."): There is no largest cardinal.

Proof: Let $\kappa$ be a supercompact cardinal, and suppose that there were a largest cardinal $\lambda$. Since $\kappa$ is a cardinal, $\lambda \geq \kappa$. By the $\lambda$-supercompactness of $\kappa$, let $j: V \rightarrow M$ be an elementary embedding into an inner model $M$ with critical point $\kappa$ such that $M^{\lambda} \subseteq M$ and $j(\kappa) > \lambda$. By elementarity, $M$ thinks that $j(\lambda) \geq j(\kappa) > \lambda$ is a cardinal. Then since $\lambda$ is the largest cardinal, $j(\lambda)$ must have size $\lambda$ in $V$. But then since $M$ is closed under $\lambda$ sequences, it also thinks that $j(\lambda)$ has size $\lambda$. This contradicts the fact that $M$ thinks that $j(\lambda)$, which is strictly greater than $\lambda$, is a cardinal.