Q1: A very simple example is given in Grothendieck's Tohoku paper "Sur quelques points d'algebre homologiquie", sec. 3.8. Edit: The space is the complement of plane, and the sheaf is constructed by using a union of two irreducible curves in the plane, intersecting at two points. The sheaf is a constant sheaf.
Q2: Cech cohomology and derived functor cohomology coincide on a Hausdorff paracompact space (the proof is given in Godement's "Topologie algébrique et théorie des faisceaux"). Are you looking for I don't know of an example on a non paracompact space ?where they differ.

