Hoffman and Singleton proved that a regular graph with girth 5 and diameter 2 has to have degree 2, 3, 7, or 57. If I recall correctly, the proof used spectral properties of the adjacency matrix to produce some non-polynomial equation for which these were the integer solutions.
There are unique examples of the first three cases: degree 2 is a pentagon, degree 3 is the Petersen graph, and degree 7 is the Hoffman-Singleton graph. The existence of the degree 57 graph is still open (as far as I know).
