Since $u$ tends to $0$ as $x$ goes to infinity and it is apparently supposed to be $C^1$, it is bounded. Since the Laplace operator is a negative operator, the operator $L = \Delta - u$ is bounded from above, meaning $(Lu, u) \leq C$ for some constant constant $C$. Here, one can choose $C:= \min u$. It is a classical theorem that such operators, when densely defined, have a self-adjoint extension (this can be found in many books, if necessary I can give a citation). In this case, a dense domain would be the set of Schwartz functions, for example, and the theorem states that $L$ is essentially self-adjoint here.
Now, the spectrum of a self-adjoint operator is real, and clearly also bounded from above by $C$.
Regarding eigenvalues, however, I am afraid that your operator is not very well conditioned in general. If $u$ is a positive function, then there will be no eigenvalues at all, except possibly zero. However, if $C = \min u < 0$, then there can be eigenvalues in the interval [0, C], but this is not necessary.
To give some explanation, there is a theorem that states in your case, if $u$ tends to $0$ when $x \rightarrow \infty$, then the essential spectrum is bounded from above by $0$.
So, there is in general no reason, why this solution should have any solution in $L^2$, but if it does, it automatically fulfills your condition ii, as does every function in $L^2$. Also, as far as I know, there is little to no hope to write down any solution analytically.